A simple fluorometrical assay of histidine decarboxylase activities in rat tissues.
نویسندگان
چکیده
منابع مشابه
Spectroscopic analysis of recombinant rat histidine decarboxylase.
Mammalian histidine decarboxylases have not been characterized well owing to their low amounts in tissues and instability. We describe here the first spectroscopic characterization of a mammalian histidine decarboxylase, i.e. a recombinant version of the rat enzyme purified from transformed Escherichia coli cultures, with similar kinetic constants to those reported for mammalian histidine decar...
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Mammalian ornithine decarboxylase (ODC) is a very unstable protein which is degraded in an ATP-dependent manner by proteasome 26S, after making contact with the regulatory protein antizyme. PEST regions are sequences described as signals for protein degradation. The C-terminal PEST region of mammalian ODC is essential for its degradation by proteasome 26S. Mammalian histidine decarboxylase (HDC...
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Hydrocortisone-stimulated synthesis of histidine decarboxylase (EC 4.1.1.22) was studied in rat stomach. Single or daily administration of hydrocortisone increased the histidine decarboxylase activity but not the histamine content in the fundic glands of stomachs from fed and fasted rats with or without adrenalectomy, and also in those from adrenalectomized fasted rats with concomitant treatmen...
متن کاملCoexistence of adenosine deaminase, histidine decarboxylase, and glutamate decarboxylase in hypothalamic neurons of the rat.
Neurons immunoreactive for the enzyme adenosine deaminase (ADA) in the posterior basal hypothalamus of the rat have a distribution pattern similar to those immunoreactive for histidine decarboxylase (HDC) and are particularly numerous in the tuberal (TM), caudal (CM) and postmammillary caudal (PCM) hypothalamic magnocellular nuclei which harbor neurons containing glutamic acid decarboxylase (GA...
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epoxyquinomicins A, B, C, and D, respectively. Therefore, these epoxyquinomicins are unique candidates for antiinflammatory agents. The modeof action appears to be different from that of common NSAIDs(non-steroidal antiinflammatory drugs)3), since epoxyquinomicins C and D did not inhibit cyclooxygenase-1 at the concentration of 300 /iu. The mechanism of the antiarthritic effect has not been elu...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1993
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)51214-8